【摘要】 采用循环伏安(CV)法和电化学发光(ECL)法,研究了苦参碱(Matrine,MT)与联吡啶钌(Ru(bpy)23+)体系的电化学行为和电致化学发光(ECL)行为,以金电极为工作电极,建立了测定MT的ECL分析新方法。研究结果表明,在0.1 mol/LNa2HPO4-NaH2PO4缓冲溶液中,当扫描速度为0.1V/s时,该ECL的峰高与MT在1.5×10-7～1.5×10-4 mol/L范围呈线性关系(r=0.9989),检出限(S/N=3)为7.3×10-9 mol/L。平行测定1.5×10-5 mol/L的MT溶液10次,发光强度值的相对标准偏差(RSD)为4.01%。对样品进行回收率试验,加入回收率在97.1%～104.0%之间,RSD为2.86%(n=5)。该方法具有较高的选择性和灵敏度,样品处理简单快速,可用于药物中苦参碱的测定。更多还原

【Abstract】 The electrochemilumine scence(ECL) intensity of Ru(bpy)2+3could be enhanced in the presence of matrine and an ECL method was therefore established for determination of the matrine at gold electrode.The electrochemical and ECL behaviors of this system were studied.In PBS buffer solution(pH=8.0),the ECL peak counts increased linearly with the increase of the concentration of matrine in the range of 1.5×10-7-1.5×10-4 mol/L at scanning rate of 100 mV/s with a linear correlation coefficient of 0.9989.The detection limit for matrine was 7.3×10-9 mol/L and the RSD for 10 replicate determinations of 1.5×10-5mol/L matrine was 4.01%.The results of recovery tests indicated that the recovery was 92.5%-101.4% and the RSD was 2.86%(n=5).The proposed method showed high selectivity,sensitivity and simplicity,and was applied to the analysis of matrine tablet with satisfactory results.更多还原