A MS method for casein allergen analysis was developed to reduce the restriction of protein extraction and digestion from tannins in red wine.Unique selective reaction monitoring(SRM) transitions were selected and confirmed for qualitative and quantitative detection of α-S1,α-S2 and β-caseins.Effective extraction and fast digestion of red wine protein were realized by utilizing polyvinylpolypyrrolidone(PVPP),which also reduced the pre-treatment time within 2 h.Results showed that the PVPP method increased r...
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