【摘要】 基于8-17 DNA酶的催化切割特性和类辣根过氧化物酶DNA酶的氧化还原反应催化特性,发展了一种新型核酸酶催化放大传感体系,并用于Pb2+的比色检测。考察了K+浓度,pH值及反应时间对检测体系的影响。ABTS吸光度变化与Pb2+浓度在5.0～100 nmol/L范围内呈良好的线性关系,检出限为3.0 nmol/L。此外,因Pb2+酶的特异性,本方法对Pb2+具有良好的选择性。更多还原

【Abstract】 Based on the catalytic cleavage of 8-17 DNA enzyme(DNAzyme) and the catalytic redox of horseradish peroxidase(HRP)-mimicking DNAzyme,a novel DNAzyme catalytic amplification biosensing platform has been proposed for the colorimetric detection of lead ions(Pb2+).The effects of K+ concentration,pH value and incubation time on the detection system were investigated.The system exhibited a dynamic response range for Pb2+ from 5 to 100 nmol/L with a detection limit of 3 nmol/L.In addition,the selectivity of the sensor for Pb2+ against other environmentally related metal ions was outstanding.更多还原