【摘要】 目的:研究sCD40L、IL-10在糖尿病及糖尿病微血管病变发生发展中的作用及其相关性。方法:选取2009年6月～2009年12月在我院内分泌科住院的2型糖尿病(DM2)患者(糖尿病无微血管病变组30例,糖尿病微血管病变组60例),健康对照组30例,采用ELISA测定患者血清sCD40L、IL-10(试剂盒购自上海西唐生物科技有限公司),并同时用血清采用酶学比色法测定血脂,采指尖血用糖化血红蛋白分析仪测定糖化血红蛋白(HbA1c)。结果:①DM2患者的HbA1c和TG高于正常对照组(P<0.05),DM2患者的TC、HDL、LDL和正常对照组无统计学差异(P>0.05),糖尿病微血管病变组的HbA1C、TC、TG、LDL、HDL和糖尿病无微血管病变组无统计学差异。②sCD40L糖尿病无微血管病变组高于正常对照组,糖尿病微血管病变组高于无微血管病变组。③IL-10水平糖尿病无微血管病变组低于正常对照组,糖尿病微血管病变组低于无微血管病变组。④sCD40L与IL-10呈负相关。结论:随着糖尿病病情的进展sCD40L水平逐渐升高,IL-10逐渐减低。更多还原

【Abstract】 Objective To study the correlativity between serum sCD40L,IL-10 and occurrence as well as development of diabetic microangiopathy.Methods The treatment of patients with type 2 diabetics who were hospitalized at endocrine division from June 2009 to December 2009 was chosen as study object.The study included 30 cases for the groups of non-diabetic microangiopathy,60 cases for the groups of diabetic microangiopathy and 30 cases for the groups of the healthy control,testing serum sCD40L and IL-10 by ELISA,while the serum was used for determination of blood lipid with zymology colorimetric method,and fingertip blood was collected to measure glycated hemoglobin by glycated hemoglobin analyzer.Results(1) There was no statistical difference(P>0.05)in ages and sexes of the groups;HbA1c and TG of the type 2 diabetes were significantly higher than these in normal controls(P<0.05),and there were no statistical difference in the TC,HDL,LDL of the type 2 diabetics compared with normal controls(P>0.05),and no statistical difference in HbA1c,TC,TG,LDL and HDL of diabetes mellitus with diabetic microangiopathy compared to the groups of non-diabetes microangiopathy.(2) sCD40L non-diabetes microangiopathy group was significantly higher than control group,diabetes microangiopathy was obviously higher than group of diabetes without microangiopathy.(3) IL-10 level of non-diabetes microangiopathy group was significantly lower than control group,diabetes microangiopathy showed prominently lower than group of diabetes without microangiopathy.(4) sCD40Lwas negatively correlated with IL-10.Conclusion Along with the progress of pathosis in diabetes,sCD40L level was rising and IL-10 gradually declined更多还原