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不同处理方法对血浆组织型纤溶酶原激活剂活性的影响
Influence on activity of tissue plasminogen activator in plasma sample with different disposal
【摘要】 目的比较不同处理方法对血浆组织型纤溶酶原激活剂(t-PA)活性的影响。方法检测3种不同的滤纸条(Whatman#1、Periopaper、国产滤纸)对血浆中t-PA酶活性的洗脱率,及观察收集血浆样本的Periopaper滤纸条在两种不同处理方法 (洗脱、非洗脱)、不同温度(-20℃、-80℃)、不同时间(1、2、4、12周)t-PA酶活性的保持情况。结果 3种滤纸条中,Periopaper对t-PA活性的洗脱率最高,Whatman#1次之,国产滤纸最差;在未洗脱状态下,-20℃和-80℃保存12周,t-PA活性几乎不受影响,而洗脱状态对t-PA活性影响较大。结论 Perio paper是理想的收集t-PA活性的滤纸条,未经洗脱并在-80℃状态下保存时t-PA活性能稳定保持3个月。
【Abstract】 Objective To research the influence on the activity of tissue plasminogen activator(t-PA)in plasma sample with different disposal.Methods The elution efficiency of plasma t-PA activity were measured from three kinds of collection paper:whatman#1,periopaper,domestic filter paper.Periopapers soaked with plasma were divided into two groups.One group was treated eluting beforehand,another group was treated no eluting beforehand.Half samples of each group were stored at 20℃ and the rest were stored at -80℃.The activity of t-PA of each sample were measured in 1week,2weeks,4weeks,and 12weeks.Results The results showed that periopaper had the highest elution efficiency of t-PA activity.In the condition of no-eluting before hand,the t-PA activity of the samples stored in -20℃ and -80℃ had no significant change within three months.Conclusion This study indicates that the periopaper is the ideal filter paper to collect GCF and the t-PA activity can retain at least three months in the condition of no eluting beforehand from strip paper with -80℃.
- 【文献出处】 福建医药杂志 ,Fujian Medical Journal , 编辑部邮箱 ,2013年04期
- 【分类号】R446.8
- 【下载频次】62