【摘要】 UGPase(UDP-glucose pyrophosphorylase,尿苷二磷酸葡萄糖焦磷酸化酶)是糖代谢过程中的关键酶,对蔗糖积累和细胞壁形成有重要影响.将处于生长期的甘蔗叶片经蔗糖、光照、冷诱导等条件处理后,测定UGPase酶活性,并利用real-time PCR技术同步测定Ugp的相对表达量.研究结果表明:环境中的蔗糖、低温(4℃)、光照对UGPase基因的表达调控呈上调,对UGPase酶活性有提高的作用.更多还原

【Abstract】 UDP-glucose pyrophosphorylase( UGPase) is the key enzyme in the sucrose metabolism,and it plays an important role in sucrose accumulation and the formation of a new cell wall. After the leaves of sugarcane in the growth period are treated in the condition of sucrose,light, low-temperature,relative expression of the UGPase gene in the leaves of sugarcane was determined by real-time PCR. And UGPase activity was determined at the same time. The results of the study show that the UGPase gene was profoundly up-regulated by feeding sucrose to excised leaves and by an exposure of plants to low tempreture( 4 ℃). The UGPase activity also increased under conditions of sucrose feeding and exposure to cold.更多还原