【摘要】 在本实验室之前合成的多表位抗原基因pcxz的基础上,将之3次串联重复构建了3pcxz,以重组表达的3PCXZ作为抗原,HCV病人血清为检测抗体,作Western blot杂交,结果表明3PCXZ抗原能被HCV阳性血清特异识别,而同正常人的血清无反应.通过对BALB/c小鼠的免疫应答进行检测,发现合成的多表位抗原3PCXZ诱导BALB/c小鼠产生的IgG抗体滴度为5.8×105;IgG亚型分析发现,这一免疫反应主要是基于TH2方式,在细胞免疫应答方面亦能激活特异的CD4+T辅助细胞CTL效应,最高溶解率达到40.54%,在1×106脾淋巴细胞中最多可以产生(698±229)个分泌INF-γ的CD4+T细胞.更多还原

【Abstract】 Based on the multi-epitope antigen gene pcxz which is against the hepatitis C virus synthesized before,this research triplicate pcxz into 3pcxz.The antigenic specificity of 3pcxz was determined by recognizing antibodies in serum samples from hepatitis C virus patients,but not from healthy subjects or subjects who had the hepatitis B virus.The characteristics of 3PCXZ immunogenicity were evaluated in BALB/c mice.Strong antibody responses were generated in mice immunized with either naked 3PCXZ or 3PCXZ in Freund’s adjuvant.Analysis of antibody subtype shows this response is mainly through the Th2 pathway.It can also stimulate the CTL effects of specific CD4+T help cell,the lytic activity of cytotoxic T lymphocytes can reach 40.54%,3PCXZ can also significantly increased interferon-g secretion from CD4+T cell,and 698±229 interferon-g-secreting CD4+ can be produced in 1×106 spleen lymphocytes.更多还原