Objective To explore the induction of L-forms of Mycobacterium abscessi using isoniazid. Methods Mycobacterium abscessi were cultured in aqueous culture media in the presence or absence of 128 μg/ml isoniazid. The culture media containing isoniazid were filtered with 0.45 μm membrane, and the filtrate was subcultured in nutrient agar media for reversion. The isoniazid-free and isoniazid-containing media and the reversion bacteria were observed for cell wall integrity by cell wall staining and transmission e...