【摘要】 目的探讨胃泌素对大鼠小肠上皮1,25(OH)2D3膜相关快速反应类固醇结合蛋白(1,25D3-MARRS结合蛋白)表达的影响。方法应用免疫组织化学、RT-PCR和免疫印迹法,检测胃泌素、奥美拉唑处理后的SD大鼠小肠中1,25D3-MARRS结合蛋白的表达情况与对照组的差异,并测大鼠血清中钙、磷离子的浓度变化。结果大鼠小肠上皮细胞中1,25D3-MARRS结合蛋白分布于胞核、胞浆和胞膜。胃泌素、奥美拉唑均引起大鼠小肠组织1,25D3-MARRS结合蛋白的mRNA和蛋白两个水平表达增高(P<0.05),但其作为一个促小肠钙磷吸收的蛋白,无明显增高血清钙磷浓度的作用(P>0.05)。结论 1,25D3-MARRS结合蛋白作为一个广泛分布的多功能蛋白,受胃泌素的调节,在消化道相关疾病具有很高的研究价值及临床应用前景。更多还原

【Abstract】 Objective To explore the effects of gastrin on the expression of 1,25(OH)2D3 - membrane associated rapid response steroid (1,25D3 - MARRS) binding protein in rat intestinal epithelium. Methods SD rats received intraperitoneal injections of gastrin, omeprazole or physiological saline. The protein expression of 1,25D3 -MARRS binding protein in SD rat intestinal was determined with Western blotting and immunohistochemistry, and its mRNA levels determined by RT- PCR. The serum calcium and phosphate levels in the rats were also detected. Results Immunohistochemistry showed that 1,25D3 - MARRS binding protein was expressed mainly in the nuclei, cytoplasm and membrane of the intestinal epithelial cells. Both the protein and mRNA expression levels of 1,25D3 - MARRS binding protein were up- regulated after treatments with gastrin and omeprazole (P<0.05), but the serum calcium and phosphate concentrations showed no obvious increase. Conclusion 1,25D3 MARRS binding protein, which is widely expressed with versatile functionalities, is regulated by gastrin and shows high potentials in the study of gastrointestinal diseases.更多还原