【摘要】 目的探讨抑癌基因WTX启动子区域甲基化水平及其在胃癌中的作用。方法运用MassARRAY定量分析系统分析20例胃癌及配对正常组织和3种胃癌细胞株(MGC803、SCG7901和BGC823)中WTX基因启动子区域甲基化水平。应用5-杂氮-2’-脱氧胞苷(5-aza-dC)对胃癌细胞BGC823进行去甲基化处理,分析其对WTX基因启动子区域甲基化水平的影响。结果在胃癌及其配对正常组织和胃癌细胞株中,WTX基因启动子区域甲基化水平普遍较低,并且3组之间无统计学差异。用5-aza-dC处理后并不能改变胃癌细胞株中WTX基因启动子区域的甲基化水平。结论胃癌中基本不存在WTX基因启动子区域高甲基化状态。更多还原

【Abstract】 Objective To investigate the changes in methylation levels of the promoters of the tumor suppressor gene Wilms’ tumor gene on the X-chromosome(WTX) and its possible role in gastric cancer.Methods WTX promoter methylation levels were detected in 20 pairs of specimens of gastric cancer and matched normal tissues and in 3 gastric cancer cell lines(MGC803,SCG7901,and BGC823) using the Sequenom MassARRAY quantitative analysis system.The gastric cancer cell line BGC823 was treated with 5-aza-2’-deoxycytidine(5-aza-dC) for demethylation and the changes in the level of WTX promoter methylation were investigated.Results WTX promoter methylation levels were very low and showed no significant differences among normal gastric tissues,gastric cancer tissues and the 3 gastric cancer cell lines.In BGC823 cells,treatment with 5-aza-dC did not obviously affect the promoter methylation levels of WTX.Conclusion High methylation levels of WTX promoters are rare in gastric cancer.更多还原