Objective To construct an eukaryotic expression vector encoding human Bcl-2 and adenovirus E1B 19 kDa interacting protein(BNIP3) gene,and to investigate the effect of BNIP3 over-expression on the chemosensitivity of human colon cancer cell line HT-29.Methods The full-length cDNA of BNIP3 was amplified by PCR and cloned into pEGFP-C3 vector using genetic engineering technology.The recombinant expression vector pEGFP-C3 / BNIP3 was confirmed by enzyme digestion and sequencing,and then was transferred into HT-...
【基金】
重庆市自然科学基金重点项目(CSTC2007BA5008)~~
【更新日期】
2013-09-29
【分类号】
R735.35
【正文快照】
Supported by the Key Project of Natural Science Foundation of Chongqing(CSTC2007BA5008).Corresponding author:Liang Houjie,E-mail:Lianghoujie@sina.com结肠癌是一种常见的消化系统恶性肿瘤。化疗是进展期结肠癌最基本的治疗手段,但是肿瘤细胞对化疗的耐药严重影响了?