【摘要】 以独一味叶片为材料,采用RT-PCR和RACE方法克隆了独一味苯丙氨酸解氨酶基因(PAL)的全长cDNA,命名为LrPAL基因。测序结果表明,LrPA L基因全长2 298bp,含有1个2 145bp的完整开放阅读框(ORF),编码714个氨基酸。蛋白序列分析表明,其包含典型的PAL活性中心序列(GTITASGDLVPLSYIA),与其他植物的PAL蛋白有很高的同源性。系统进化树分析表明,独一味LrPAL与唇形科植物的PAL蛋白聚为一类,说明两者的亲缘关系较近。用Real-Time PCR方法检测发现,LrPAL基因在独一味的叶中表达量最高,茎中表达量最少。研究结果推测,从独一味中克隆获得的苯丙氨酸解氨酶基因(LrPAL)是典型的PAL家族成员,在独一味各组织发育过程中具有重要功能。更多还原

【Abstract】 In this study,aphenylalanine ammonia-lyase(PAL)gene was cloned fromLamiophlomis rotata by the methods of Reverse-Transcription PCR and RACE.The full-length cDNA of L.rotata PAL(designated as LrPAL)was 2 298bp,and contained a complete open reading frame(ORF)of 2 145bp,which encoded 714amino acid residues.Sequence analysis showed that it contains the typical PAL active site sequence(GTITASGDLVPLSYIA).Homology analysis indicated that the deduced LrPAL protein was highly homologous to other PAL proteins from different plant species.Phylogenetic tree analysis revealed that LrPAL had closer relationship with PALs from Labiatae plants than that from other plants.The results from Real-Time PCR indicated that the expression of LrPALgene was the strongest in the leaves of L.rotata,and the least in shoots.As a result,the gene LrPALwhich was cloned and characterized fromL.rotatais a typical member of PAL family.The result indicated that LrPAL gene played a important role in the development of L.rotataplant.更多还原