【摘要】 目的观察大豆异黄酮载药纳米颗粒对肝纤维化大鼠的抗过氧化作用。方法采用四氯化碳灌胃法建立肝纤维化大鼠模型,随机分成对照组、肝纤维化组、空白纳米颗粒组(SPIO组)、大豆异黄酮组(SI组)、载药纳米颗粒组(SI-SPIO组),每组24只。对照组给予生理盐水尾静脉注射,其他各组除四氯化碳灌胃外,分别给予生理盐水尾静脉注射、SPIO、大豆异黄酮和SI-SPIO载药纳米颗粒尾静脉注射治疗,观察对照组和治疗组之间肝脏生化指标、过氧化损伤指标和肝脏组织学变化。结果 8周后对各组大鼠的肝脏生化指标和肝纤维化程度进行评估,SI组和SI-SPIO组明显优于肝纤维化组和SPIO组;过氧化指标检测,与对照组相比,肝纤维化组和SPIO组MDA明显升高(2.45±0.77,2.69±1.01,P<0.05),GSH-Px和SOD明显降低(300.74±16.25,299.18±15.57和409.77±20.46,431.56±19.25,P<0.05);与肝纤维化组相比,SI组和SI-SPIO组MDA明显降低(0.85±0.12,0.90±0.02,P<0.05),GSH-Px和SOD明显升高(340.25±11.85,366.45±14.83和467.27±16.11,485.75±22.54,P<0.05)。SI-SPIO组过氧化指标较SI组改善更为明显(P<0.05)。结论大豆异黄酮具有抗氧化作用,能够抑制大鼠肝纤维化形成,载药纳米有助于药效的发挥。更多还原

【Abstract】 Objective To investigate the anti-peroxidative effects of SPIO nanoparticles as a drug delivery system when combined with soybean isoflavones( SI-SPIO). Methods The liver fibrosis models of rats were established by CCL 4 gavage. 120 rats were random grouped as control,fibrosis,SPIO tail vein injection,isoflavone treatment and SI-SPIO treatment group. Normal saline injections were given in control group,CCL 4 gavage were given in other groups,as well as NS injection,SPIO,SI and SI-SPIO injections to 24 rats of each group,respectively. Biochemical and histological indicators were analyzed. Peroxidative indicators were detected as well. Results Liver fibrosis was significantly improved in SI and SI-SPIO groups vs. control both in biochemical and histological aspects. Peroxcidative indicators such as MDA increased in liver fibrotic and SPIO groups( 2. 45 ± 0. 77,2. 69 ± 1. 01,P < 0. 05),GSH-Px and SOD decreased( 300. 74 ± 16. 25,299. 18 ± 15. 57 and 409. 77 ± 20. 46,431. 56 ± 19. 25,P < 0. 05),SI administration reduced MDA( 0. 85 ± 0. 12,0. 90 ± 0. 02,P < 0. 05),increased GSH-Px and SOD in SI and SI-SPIO groups( 340. 25 ± 11. 85,366. 45 ± 14. 83 and 467. 27 ± 16. 11,485. 75 ± 22. 54,P < 0. 05). SI-SPIO group presents much more efficiency comparing with SI group( P < 0. 05). Conclusion SI has anti-peroxidative effect and inhibits liver fibrosis in rats. SPIO nanoparticles make the effects more efficient as a drug delivery system.更多还原