【摘要】 目的探讨肿瘤坏死因子α(TNF-α)-308G/A基因多态性与儿童紫癜性肾炎(HSPN)的关系。方法应用聚合酶链反应(PCR)扩增产物直接测序方法对110例过敏性紫癜(HSP组)汉族儿童进行TNF-α-308G/A基因型分析,包括紫癜性肾炎52例(HSPN组),无合并肾炎58例(单纯HSP组)。酶联免疫吸附法检测血浆TNF-α水平。90例健康汉族儿童作为对照组。结果 HSP组TNF-α-308基因型分布频率与对照组比较差异无统计学意义(P>0.05)。HSPN组GA基因型(29%)、A等位基因频率(18%)均明显高于单纯HSP组(分别为10%和7%,P<0.05),其中GA+AA基因型患儿血浆TNF-α水平(7.1±2.3 pg/mL)较GG基因型患儿(5.7±1.5 pg/mL)明显升高(P<0.05)。结论 TNF-α-308G/A基因多态性与HSP儿童肾脏损害的发生有关,A等位基因可能是儿童HSPN的易感基因。更多还原

【Abstract】 Objective To study the relationship of tumor necrosis factor-alpha(TNF-α)-308G/A gene polymorphisms with Henoch-Schnlein purpura nephritis(HSPN) in children.Methods Using the direct DNA sequencing method,polymorphisms in the TNF-α promoter region(-308) were genotyped in 110 Han children with Henoch-Schnlein purpura(HSP group),including 52 children with nephritis and 58 children without nephritis.Plasma TNF-α levels were measured using ELISA.Ninety ethnically matched healthy children were used as the control group.Results There were no significant differences in the polymorphisms of TNF-α(-308G/A) between the HSP and control groups(P>0.05).The GA genotype(29% vs 10%) and A allele frequency(18% vs 7%) in HSP children with nephritis(HSPN) were more common than in those without nephritis(P<0.05).Plasma TNF-α levels in HSPN children with GA+AA genotype(7.1±2.3 pg/mL) were significantly higher than those with GG genotype(5.7±1.5 pg/mL)(P<0.05).Conclusions TNF-α-308GA genotype and A allele may contribute to the increased risk for the development of nephritis in children with HSP.更多还原