【摘要】 以低磷胁迫处理的野生大豆w343叶片为材料,根据大豆的GmPAP1基因设计一对特异引物,采用同源克隆的方法获得了野生大豆紫色酸性磷酸酶PAP1基因的cDNA序列,并将其命名为GsPAP1。该基因与已报道的大豆GmPAP1基因长度一致,均为999 bp,编码332个氨基酸,分子量为37.71 kD,等电点为7.38。但在cDNA的编码区共有9处发生了碱基的替代,其中有5处转换和4处颠换,有7处编码的氨基酸残基不同,其序列一致性达99.38%。系统进化树分析表明,野生大豆GsPAP1基因与大豆、羽扇豆、甘薯、葡萄等植物的PAP1基因有较高同源性。更多还原

【Abstract】 In this study,PAP1 gene was cloned from wild soybean w343 planted under deficient-Pi condition with designed primers according to the sequence of GmPAP1 gene from cultivated soybean based on homologous cloning method and named GsPAP1.The cloned GsPAP1 gene was 999 bp in size and encoded 332 amino acids as same as GmPAP1 gene from soybean,with molecular weight of 37.71 kD and isoelectric point 7.38.Sequence alignment of GmPAP1 with GsPAP1 showed that there were 9 single nucleotide polymorphisms,including 5 locus transitions and 4 locus transversion.GsPAP1 had higher homology to GmPAP1 with the identities of 99.38%.Amino acid sequence alignment showed that 7 deduced amino acids of GsPAP1 were different from GmPAP1.The phylogenic tree results showed that GsPAP1 gene from w343 shared high similarities to the previously reported PAP1 genes from other plants,such as Glycine max,Lupinus luteus,Ipomoea batatas,Vitis vinifera and so on.更多还原