The mature peptide coding sequence of xylanase gene xynB(746 bp)was amplified from the Aspergillus niger CICC2462,for high expression of glucoamylase gene locus in Aspergillus niger CICC2462, Splicing xynB gene fragment, glucoamylase gene 5 'homology arm and 3' homologous arm for homologous recombination expression cassette GBG by overlap extension PCR,The whole gene was inserted into expressional vector pSZH-CYM form the Aspergillus niger expressional vector pSZH-xynB, Using freeze thawing method pSZH-xynB...
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