According to the sequence of asparagine synthetase(AS-B) gene in soybean(GenBank accession number: GMU55874),a pair of specific primers was designed to obtain the AS-B gene from Glycine max by PCR amplification using cDNA as template.The gene of AS-B was then cloned into prokaryotic expression vector pET30a(+),constructing a recombinant expression vector pET30a-AS,which was introduced into E.coli Rosetta(DE3) pLysS.After the optimization of induced conditions,recombinant AS-B was expressed in a soluble form...
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