【摘要】 病程相关蛋白(PR)在植物对病原物的防卫反应过程中具有重要作用。从桑树叶片中克隆得到桑树病程相关蛋白基因MuPR1a(GenBank登录号:KC453993)。该基因编码区全长为516 bp,共编码171个氨基酸,编码蛋白质具有一个典型的分泌型信号肽序列和多个PRs蛋白保守结构域,其氨基酸序列与其它植物的PR1a蛋白具有很高的同源性。利用巢式PCR(Tail-PCR)技术克隆得到MuPR1a的启动子,并将其命名为MPRp(GenBank登录号:KC849423),该启动子序列含有CAATbox、TATA-box、TCA-element、GCC box等正常转录起始必需的顺式作用元件。农杆菌介导的烟草叶片瞬时表达分析显示,MPRp具有启动子的功能,能够驱动下游报告基因的表达并且具有一定的被病原菌诱导表达的活性。更多还原

【Abstract】 Pathogenesis-related proteins( PR) play a key role in plant defense response against pathogens. In this study,mulberry pathogenesis-related protein gene MuPR1a( GenBank accession No. KC453993) was cloned from mulberry leaves. The full length of its coding region is 516 bp and encodes 171 amino acids. MuPR1a possesses one typical secretory signal peptide and multiple conserved domains of PR proteins. Sequence comparison analysis showed that MuPR1a had very high sequence identity with RP1a protein from other plants. The promoter of MuPR1a gene was cloned using TailPCR technique and was designated as MPRp( GenBank accession No. KC849423). MPRp contains cis-acting elements CAAT-box,TATA-box,TCA-element,and GCC box,which are necessary for the normal transcriptional initiation. The activity of MPRp was investigated by means of Agrobacterium-mediated transient expression in tobacco leaves,and the result indicated that MPRp could drive the expression of reporter gene and could be induced upon infection of pathogen.更多还原