【摘要】 设计了一种基于氧杂蒽结构的"Turn-Off"型荧光探针(P3),在生理pH条件下对磷酸根(Pi)较好的荧光淬灭性能,在与其它竞争性含氧阴离子(硫酸根、硝酸根、碳酸氢根)以及其它常见阴离子(氟离子、氯离子、溴离子等)对该识别的竞争性影响考察中,亦显示了较好的特异性。从荧光滴定法得到荧光探针(P3)与磷酸根(Pi)形成超分子复合物的摩尔比为1∶1,复合物稳定常数K为5.28*103。通过量子化学计算表明磷酸根与!共轭体系产生了较强的相互作用,并导致P3分子平面上翘,在一定程度上破坏了原先的共面型,磷酸根上氧部位的孤对电子与!共轭体系存在着轨道交叠,即p-!共轭作用。可将此探针应用于磷酸酯类物质的检测。更多还原

【Abstract】 A xanthene structure of "Turn-Off" type fluorescent probe (P3) was designed,which showed selectively quenching response to phosphate ion (Pi) over other anion ions at the physiological pH condition.The molar ratio of Pi/P3and stability constant of the supramolecular complex was obtained as 1:1and 5.28*103,respectively from the fluorescence titration method.The quantum chemical calculation showed that phosphate ion and conjugate system of P3 have a strong interaction,and lead to the molecular plane of P3molecule upwards,to a certain extent,destroyed the original coplanarity.There was some orbital overlap between the lone pair electrons on phosphate and part of the conjugate system (HOMO) which meant p-p conjugation.This probe could be applied in detection of the phosphoric acid esters.更多还原