【摘要】 目的观察电针(EA)对阿尔茨海默病(AD)大鼠学习记忆能力及血浆中SOD、MDA的含量,大脑P53、P21表达的影响。方法选取正常SD大鼠36只,随机分成假手术组、模型组和EA组。EA组针刺"百会"、"太溪"、"足三里"穴治疗,通过Morris水迷宫检测大鼠的行为学改变、分光光度法观察大鼠血浆中SOD及MDA的水平;免疫荧光法观察大鼠大脑P53、P21的表达。结果与模型组比较:EA组逃避潜伏期缩短,在第三象限的活动时间延长,跨越原平台位置的次数增多,血浆SOD水平升高、MDA水平降低,大脑P53、P21的表达减少,差异有统计学意义(P<0.01)。结论 EA能够改善Aβ1-42诱导的AD大鼠空间学习记忆能力,可能与提高SOD活性,降低MDA含量,下调P53,P21蛋白表达有关。更多还原

【Abstract】 Objective of electroacupuncture(EA) of Alzheimer′s disease(AD) rat learning and memory ability and plasma SOD,MDA content in brain P53,P21 expression.Methods The normal 36 SD rats,were randomly divided into sham operation group,model group and EA group.EA group acupuncture "Baihui","Taixi","Zusanli" treatment,through the Morris water maze test in rats behavioral changes,spectrophotometric observation of SOD and MDA in rat plasma levels;observation of rat brain P53,P21 expression by immunofluorescence.Results Compared With the model group,group EA escape latency,longer in the third quadrant of the activities of the time,the number of crossing platform position increased,decreased the elevated plasma SOD level,MDA level,reduce the expression of brain P53,P21,and the difference was statistically significant(P<0.01).Conclusion electro-acupuncture treatment can improve the Aβ1-42-induced AD rat spatial learning and memory ability,may be lowered P53,P21 protein expression increase SOD activity and MDA.更多还原