【摘要】 目的探讨丝裂霉素C(MMC)对瘢痕疙瘩成纤维细胞(KFB)转化生长因子β(TGF-β)/Smads通路作用及机制。方法采用不同浓度的MMC作用于体外培养的KFB,通过采用RT-PCR方法,检测MMC对体外培养的KFB TGF-β1mRNA表达的影响,采用Western blot技术检测MMC作用下体外培养KFB中Smad2/3、Smad4、Smad7蛋白的表达。结果 RT-PCR方法显示:MMC干预组TGF-β1mRNA相对表达量明显低于正常对照组,从12.5μg/L MMC开始具有显著的统计学差异(P<0.05),随MMC浓度增大,MMC干预组TGF-β1mRNA相对表达量呈逐渐降低趋势。Western blot技术检测显示:12.5μg/LMMC开始对KFB中Smad7蛋白的表达具有明显增强效应(P<0.05),50μg/L MMC对Smad2/3蛋白的表达开始有减弱效应(P<0.05),而对Smad4蛋白的表达无明显变化。结论 MMC对TGF-β1mRNA的表达具有明显减弱效应;加入不同浓度的MMC能明显增加KFB中Smad7蛋白的表达,减弱KFB中Smad2/3蛋白的表达,而对Smad4蛋白的表达无变化。更多还原

【Abstract】 Objective To study the effect of mitomycin C(MMC) on transforming growth factor(TGF)-β/Smad signal passage in keloid fibroblasts(KFB) and its mechanism.Methods The different concentrations of MMC were acted on in vitro cultured KFB and their influence on the expression of KFB TGF-β1 mRNA was detected by the RT-PCR method.The Western blot technology was used to detect the expression of Smad2/3,Smad4 and Smad7 protein in the in vitro cultured KFB under MMC action.Results The RT-PCR results showed that TGF-β1 mRNA related expression quantity in the MMC intervention group was significantly lower than that in the normal control group,showing statistical differences starting from 12.5 μg/L MMC(P<0.05).With the MMC concentration increase,the related expression quantity of TGF-β1 mRNA in the MMC intervention group showed the gradually decreasing trend.The Western blot detection displayed that 12.5 μg/L MMC started to show the enhancing effect on Smad7 protein expression in KFB(P<0.05),50 μg/L MMC started the weakening effect on the expression of Smad2/3 protein(P<0.05),but had no effect on the Smad4 protein expression.Conclusion MMC has obviously decreasing effect on expression of TGF-β1 mRNA.The different concentrations of MMC can obviously increase the expression of Smad7 protein,decrease the expression of Smad2/3 protein in KFB and has no effect on the expression on Smad4 protein.更多还原