【摘要】 目的探讨人脐带间充质干细胞(HUC-MSCs)治疗肝硬化大鼠转化生长因子β1(TGF-β1)和肝细胞生长因子(HGF)的表达及机制。方法 40%CCl4大豆油腹部皮下注射制备肝硬化大鼠模型,将肝硬化大鼠随机分成模型组、生理盐水组(NS组)、移植组,每组8只,另设8只作为对照组。移植前、移植后3周分别用Western blot和ELISA法检测大鼠肝组织和血清TGF-β1和HGF水平。同时于光镜下观察肝纤维化程度,计算肝纤维化组织学半定量计分。结果模型组较对照组大鼠肝组织和血清TGF-β1明显升高,HGF明显降低(P<0.05);移植组较模型组TGF-β1明显降低,HGF明显升高(P<0.05)。光镜下,模型组肝组织破坏,假小叶形成;移植组,肝纤维化明显改善。模型组大鼠肝纤维化组织学半定量计分较对照组明显增高(P<0.05);移植组较模型组计分明显降低(P<0.05)。结论 HUC-MSCs移植后可通过下调TGF-β1及上调HGF的表达,改善受损的肝组织。更多还原

【Abstract】 Objective To investigate transforming growth factor-β1(TGF-β1)and hepatocyte growth factor(HGF) expression and mechanism in liver cirrhosis rats after human umbilical cord mesenchymal stem cells(HUC-MSCs) transplantation.Methods The liver cirrhosis rates were given 40%CCl4 subcutaneous injection in abdomen to make the liver cirrhosis rats model,and then liver cirrhosis rats were randomly divided into model group,NS group and transplantation group,each group with 8 rats,and 8 rats were as control one.Three weeks before and after transplantation,TGF-β1 and HGF levels in the serum and liver tissue were tested by Western blot and ELISA.We tested liver fibrosis degree by microscopy and calculated scores of liver fibrosis.Results TGF-β1 levels in serum and liver tissue were significantly higher in model group than those in control one,however,HGF level was significantly lower(P<0.05).TGF-β1 level in transplantation group was significantly lower than that in model group(P<0.05).But HGF levels were significantly increased(P<0.05).In model group,liver tissues were destruction,false flocculus was formed.Liver fibrosis significantly decreased.Liver fibrosis in model group was significantly higher than that in control group(P<0.05),in transplantation group,which was significantly lower than that of model group(P<0.05).Conclusion The HUC-MSCs transplantation can improve impaired liver tissue by downregulating TGF-β1 and upregulating HGF.更多还原