节点文献
HPLC法测定痹痛贴中蛇床子素与二氢欧山芹醇当归酸酯的含量
Determination of Osthole and Columbianadin in Bi-Tong Paste by HPLC
【摘要】 目的:建立痹痛贴中蛇床子素与二氢欧山芹醇当归酸酯的含量HPLC分析方法。方法:采用ChromstarTMC18(4.6×250 mm,5μm)色谱柱,以乙腈-水(60∶40)为流动相,流速1.0 mL/min,检测波长330 nm,柱温30℃。结果:蛇床子素进样量在0.149~1.192μg线性关系良好,平均回收率为97.17%,RSD=0.24%(n=6);二氢欧山芹醇当归酸酯进样量在0.054~0.432μg线性关系良好,平均回收率为95.98%,RSD=0.77%(n=6)。结论:该方法简单、重复性好,可用于痹痛贴的质量控制。
【Abstract】 Objective: To establish HPLC method for determination osthole and Columbianadin in "Bi-tong paste". Methods:The samples were separated at 30℃ on a ChromstarTMC18( 4. 6 ×250 ㎜,5 μm) column eluted with acetonitrile-water( 60∶40) as the mobile phase. Flow rate was set at 1. 0mL / min and the detection wavelength was set at 330 nm. Results: Osthole showed a good linear relation in the range of 0. 149 ~ 1. 192 μg. The average recovery was 97. 17% with RSD of 0. 24%( n = 6). Columbianadin showed a good linear relation in the range of 0. 054 ~ 0. 432 μg. The average recovery was 95. 98% with RSD of 0. 77%( n = 6). Conclusion:The method is accurate,simple and with good reproducibility. It can be used for the quality control of "Bi-tong paste".
- 【文献出处】 成都中医药大学学报 ,Journal of Chengdu University of Traditional Chinese Medicine , 编辑部邮箱 ,2013年03期
- 【分类号】R286.0
- 【被引频次】3
- 【下载频次】69