【摘要】 目的:研究清开灵注射液(QKL)在体外对小鼠脾T、B细胞增殖活化的影响。方法:常规制备小鼠脾细胞,观察T细胞转化实验加ConA,观察B细胞转化实验加LPS。采用MTT法观察不同浓度QKL对小鼠脾T、B细胞增殖活化的作用。结果:不同浓度的QKL(1/3200及以上)对脾B细胞的增殖具有明显的抑制作用,且呈剂量依赖性(P<0.01)。高浓度QKL(1/400及以上)对脾T细胞增殖具有抑制作用,低浓度QKL(1/800以下)对脾T细胞增殖具有促进作用(P<0.01)。结论:QKL可通过影响T、B细胞的活化增殖调节机体的免疫反应。更多还原

【Abstract】 Objective:To study the influence of Qingkailing injection(QKL) on proliferation of mouse spleen T and B lymphocytes in vitro.Methods:Concanavalin A was added to mouse spleen lymphocytes to activate T lymphocytes and LPS to activate B lymphocytes.MTT method was used to observe the effects of QKL of different concentration on mouse spleen T and B lymphocytes proliferation.Results:QKL of different concentration(1/3200 and above) could obviously inhibit proliferation of spleen B lymphocytes,and showed dose dependent(P<0.01).High concentration QKL(1/400 and above) could inhibit proliferation of spleen T lymphocytes,while low concentration QKL(below 1/800) could promote proliferation of spleen T lymphocytes(P<0.01).Conclusions:QKL can regulate immune reaction by influencing the proliferation of T and B lymphocytes.更多还原