【摘要】 目的:应用蛋白质组学技术,开展中医肝肾阴虚证证候学研究。方法:对类风湿关节炎(RA)肝肾阴虚证、RA寒湿痹阻证、正常对照共3组各12例血清,分别进行双向凝胶电泳,通过软件分别比较肝肾阴虚证组和其他两组图谱,找出共同的肝肾阴虚证差异蛋白质点,应用质谱进行鉴定。结果:对10个肝肾阴虚证共同的差异蛋白点,进行鉴定和SwissProt数据库检索,得到6个肝肾阴虚证差异蛋白,涉及蛋白酶抑制剂、代谢相关酶蛋白、分泌多肽、运输蛋白等。结论:这些蛋白具有潜在的作为RA肝肾阴虚证诊断、预后标志物或治疗靶点的意义,也为进一步研究肝肾阴虚证实质提供了依据。更多还原

【Abstract】 Objective: Proteomic technique was applied to analyze rheumatoid arthritis(RA) patients with yin deiciency of liver and kidney syndrome.Methods: Serum proteins of RA patients with yin deiciency of liver and kidney syndrome group(12cases) were compared with that of normal control group(12cases) and RA patients with cold-dampness arthralgia spasm syndrome group(12cases) respectively by two-dimensional gel electrophoresis;common differential protein spots of yin deiciency of liver and kidney syndrome were analyzed by mass spectrometry.Results: 10 common diferential protein spots were selected and 6 proteins were identiied by mass spectrometry,which related to protease inhibitor,enzyme,secreted polypeptide,transport/cargo protein,and so on.Conslusions: These proteins are of potential signiicance in the diagnosis,prognostic markers,or treatment targets of RA patients of yin deiciency of liver and kidney syndrome,and also provide a basis for further studies of the essence of syndromes in traditional Chinese medicine.更多还原