【摘要】 目的:观察清胰化积方(QYHJ)对不同EphB2表达人胰腺癌CFPAC-1裸鼠皮下移植瘤生长抑制作用差异,明确EphB2表达是否可以作为QYHJ干预有效性的预测靶点。方法:采用慢病毒介导RNAi技术建立不同EphB2表达状态人胰腺癌CFPAC-1细胞,建立裸鼠皮下移植瘤模型并以QYHJ干预4周完成相关检测。结果:QYHJ干预4周后,CFPAC-1、CFPAC-1 Con RNAi和CFPAC-1 EphB2 RNAi细胞抑瘤率分别为31.40%、31.33%和18.36%。干预后CFPAC-1 Con RNi细胞G0/G1期比例由70.77%上升至78.47%,S期细胞由22.70%降至15.17%,S期比例与0.9%氯化钠溶液(NS)组比较差异有统计学意义(P<0.05)。干预后CFPAC-1 Con RNAi凋亡细胞比例由4.77%增加至20.50%,与NS组比较差异有统计学意义(P<0.05)。结论:EphB2相对高表达的CFPAC-1细胞对QYHJ干预更为敏感,其通过降低S期细胞比例进而抑制肿瘤细胞增殖并促进凋亡而发挥抑瘤作用;QYHJ对EphB2低表达细胞生长抑制不明显;EphB2高表达可以作为是清胰化积方干预后有效性的预测指标之一。更多还原

【Abstract】 Objective: To observe the difference of tumor inhibition after Qingyihuaji Formula(QYHJ) treatment in different EphB2 expression CFPAC-1 cell for the purpose to reveal whether EphB2 expression can act as the predict target for effectiveness of QYHJ intervention.Methods: Intervention of QYHJ Formula was observed on cell growth in different levels of EphB2 expressed CFPAC-1 pancreatic carcinoma xenograft transplantation model in nude mice.Cell-cycle and apoptosis were analyzed by flow cytometry(FCM).Results: CFPAC-1,CFPAC-1 Con RNAi and CFPAC-1 EphB2 RNAi cell tumor inhibitory rate was 31.40%,31.33% and 18.36% respectively after treatment with QYHJ.QYHJ Formula significantly increased G0/G1 phase cells from 70.77% to 78.47%,and significantly decreased S phase cells from 22.70% down to 15.17% compared with NS group in CFPAC-1 Con RNAi cells(P<0.05),but CFPAC-1 EphB2 RNAi cell cycle has not changed significantly before and after intervention.Apoptosis rate was significantly increased from 4.77% to 20.50% after intervention in CFPAC-1 Con RNAi cells compared with NS group in CFPAC-1 Con RNAi cells(P<0.05).Conclusion: High expression of EphB2 cells showed sensitive to QYHJ treatment through the mechanism of inhibiting cell proliferation by blocking the cell cycle in G0/G1 phase and promoting cell apoptosis.The effectiveness of QYHJ intervention was not obvious in EphB2 low-expressing cells.EphB2 high expression can act as one predict target for effectiveness of QYHJ intervention.更多还原