【摘要】 目的:探讨逍遥散治疗肝郁脾虚证的基因调节机制。方法:25只SD雄性大鼠随机等分为5组,正常组、模型组、假手术组、α-氨基羟甲基恶唑丙酸(AMPA)组和逍遥散组。以21d慢性束缚应激方法造肝郁脾虚证模型组。假手术组、AMPA组和逍遥散组均采用21d慢性束缚联合脑部埋管微量注射方法造模,AMPA组在双侧海马CA1区埋管微量注射AMPA,逍遥散组灌服逍遥散溶液。运用RT-PCR一步法检测海马CA1区和杏仁核区AMPA受体的重要亚基GluR1 mRNA和GluR2 mRNA的表达变化。结果:在杏仁核区,与AMPA组比较,逍遥散组GluR1mRNA和GluR2 mRNA表达差异无统计学意义;在海马CA1区,与正常组比较,AMPA组GluR1 mRNA和GluR2mRNA表达差异均有统计学意义(P<0.01,P<0.05);与AMPA组比较,逍遥散组GluR1 mRNA和GluR2 mRNA表达差异有统计学意义(P<0.01)。结论:结合前期实验,从基因表达角度,进一步推断逍遥散通过纠正杏仁核和海马AMPA受体的"兴奋-抑制"失衡,重建稳态,来治疗肝郁脾虚证。更多还原

【Abstract】 Objective:To further investigate the mechanism of gene regulation of Xiaoyao Powder in the treatment of liver-stagnation and spleen-deficiency syndrome.Methods:All SD rats(n=25) were randomly divided into 5 equal groups,including the normal group,model group,sham-operation group,AMPA group and Xiaoyao Powder group.The model was established by chronic restraint for 21 days.The last 3 groups were modeled by chronic restraint for 21 days in combination with micro-injection by cerebral stereotaxis instrument.AMPA group was micro-injection with AMPA.Xiaoyao Powder group was fed with solution of Xiaoyao Powder.Results: In amygdale,compared with AMPA group,the expressions of GluR1 mRNA and GluR2 mRNA had no statistical significance in Xiaoyao Powder group.In CA1 region,compared with normal group,the expressions of GluR1 mRNA and GluR2 mRNA had no statistical significance in Xiaoyao Powder group,while had statistical significance(P<0.01,P<0.05) in AMPA group;compared with AMPA group,the expressions of GluR1 mRNA and GluR2 mRNA had statistical significance(P<0.01) in Xiaoyao Powder group.Conclusion: The regulating mechanism of Xiaoyao Powder to the expressions of subunits of hippocampus and amygdala AMPA receptor may be coincidence with that of AMPA in rat model of liver-stagnation and spleen-deficiency syndrome.It is deduced that Xiaoyao Powder cures liver-stagnation and spleen-deficiency syndrome through correcting the disorder of ’excitation-inhibition’ of hippocampus and amygdala and reconstructing the homeostasis.更多还原