【摘要】 目的了解各种血清学方法检测献血者抗-HIV的效果,探讨血清学方法的恰当及妥善应用以提高抗-HIV的准确性和检出率。方法收集广西血液中心2006年1月~2012年5月2次ELISA检测抗-HIV阳性及可疑标本972例检测结果,比较2个时间段检测结果符合性;在2次检测的基础上,再增加1次高特异性ELISA、或Determine、或明胶颗粒凝集试验(PA)、或胶体金标记法检测,以蛋白免疫印迹法(WB)做确证试验,对比与统计分析检测结果。结果献血者血液标本抗-HIV ELISA 2次检测结果的符合率为86.83%(844/972),后阶段的检测符合率91.85%(383/417)优于前阶段83.06%(461/555)(P<0.01),双试剂检测符合性优于单试剂检测(P<0.01);2次抗-HIV ELISA检测均为阳性的标本,再使用Determine、或胶体金、或ELISA、或PA试剂检测1次并阳性,其与WB确证试验阳性的符合率可分别达100%、94.96%、77.05%和58.33%;ELISA检测抗-HIV阳性的标本相应的S/CO>10者占87.90%(138/157);抗-HIV阳性者确证条带gp160和gp120的阳性率为100%,gp41和p24的阳性率均为96.86%(154/159),p55和p39阳性率为62.89%(100/159)和54.09(86/159)。结论在常规应用ELISA法检测献血者抗-HIV的基础上,恰当使用其他血清学方法可提高抗-HIV的准确性和检出率。更多还原

【Abstract】 Objective To study the effects of all kinds of serology for detection of anti-HIV in blood donors,and to explore the appropriate combination of serological methods to improve the accuracy and the detection rate of anti-HIV. Methods The specimensfrom Guangxi blood center during January 2006 to May 2013,whose results of twice anti-HIV tests via ELISA were positive were collected,and 972 cases of suspicious resultswere also collected,and the the compliances of results during two periodswere compared. On the basis of the 2 tests,we added once high specificity ELISA,or Determine,gelatin particle agglutination test( PA),or colloidal gold-labeled assay andused WB as a confirmatory test,and then all results were compared and statistically analyzed. Results The coincidence rate between the first and the second anti-HIV ELISA detection results was 87. 14%( 847 /972),but the second was 91. 85%( 383 /417) better than the first 83. 06%( 461 /555)( P < 0. 01),and double reagent for detection was better than the single reagent( P < 0. 01). For the specimens whose results of twice anti-HIV ELISA detection were all positive,we added once Determine,or colloidal gold-labeled assay,or ELISA,or PA and their results were still positive. Theirresults and WB confirmatory tests ’ positive coincidence rate can be amounted to 100%,94. 96%,77. 05% and 58. 33% respectively. Thesamples whose value of S / CO was greater than 10 in anti-HIV positiveaccounted for 87. 90%( 138 /157). In anti-HIV positive,the positive rate ofbands gp160 and gp120 was 100%,both gp41 and p24 were 96. 86%( 154 /159),p55 and p39 was lower and just 62. 89%( 100 /159) and54. 09( 86 /159) respectively. Conclusion Based on the conventional ELISA method in detection of anti-HIV in blood donors,other serological methods can improve the accuracy and the detection rate of anti HIV.更多还原