【摘要】 目的研究红细胞H抗原缺陷型血清学及分子生物学分型特点及其理化功能。方法采用血型血清学鉴定H抗原缺陷型、SSP-PCR进行ABO基因分型,采用红细胞渗透脆性试验、变形性试验检测H抗原缺陷型红细胞理化功能,模拟作为受血者或供血者进行交叉配血试验。结果 6名献血者血样经血清学方法确定为H抗原缺陷型,类孟买基因分型其中3例为h3纯合子,1例为h4纯合子,1例为h1h3杂合子,1例基因分型不确定;H抗原缺陷型红细胞渗透脆性及变形性低于H抗原正常型,H抗原缺陷型作为受血者与A型或B型红细胞主側配血凝集强度强于O型,作为供血者与O型模拟受血者主側配血凝集强度各异。结论 H抗原缺陷型红细胞与抗-H无反应,血清中能检出抗-HI/H,ABO基因正常表达;H抗原缺陷型红细胞渗透脆性及变形性低于H抗原正常型,作为受血者接受O型血液比A型或B型血液安全,作为供血者时,如果交叉配血相合时,可以输给O型受血者。更多还原

【Abstract】 Objective To study the serology,molecular biology,typing characteristics,and physical and chemical functions of H antigen-deficient red blood cells. Methods H antigen deficiency was identified by serological method,ABO and para-Bombay genotype was determined by SSP-PCR.Erythrocyte osmotic fragility test,and deformability test for H Antigen-deficient red blood cell were conducted.Cross matching tests,simulating blood recipient and donor,were also performed. Results A total of six blood donors were identified as H antigen-deficient by serological methods,among which 3 cases were h3 homozygous,one h4 homozygous,one h1h3 heterozygotes,and the last not determined.The osmotic fragility and the deformability of H antigen-deficient red blood cells were below those of H antigen normal cells.As a blood recipient,the major cross match showed stronger agglutination with A or B type RBCs,compared to O type RBCs.As a blood donor,the major cross match were negative with some recipients,and positive with others. Conclusion H antigen deficient RBCs do not react with anti-H,anti-HI/H can be detected in H deficient serum,ABO genes were normal.The osmotic fragility and the deformability of H antigen-deficient type RBCs were lower than those of H antigen normal cells.As a receipt,receiving O type blood,is safer than A type or B type blood.While as a donor,if cross match is non-reactive,H deficient RBCs could be transfused to O type recipient.更多还原