【摘要】 目的观察脑脊液淀粉样蛋白和总tau(t-tau)蛋白、磷酸tau(p-tau)蛋白等生物标志物在阿尔茨海默病(alzheimer’s disease,AD)患者和非痴呆神经疾病患者中的鉴别诊断作用。方法采用ELISA方法检测12例AD患者和22例非痴呆神经疾病患者脑脊液标本中淀粉样蛋白Aβ(1-40)、Aβ(1-42)、t-tau蛋白、p-tau蛋白的浓度水平。结果与AD患者相比,非痴呆神经疾病患者Aβ(1-42)、t-tau水平增高,Aβ(1-40)、p-tau水平降低。在诊断效果上,Aβ(1-40)、总tau蛋白曲线下面积较大,特异性、灵敏度均大于80%。与另一实验室结果比较,p-tau在两个实验室的检测结果相关性较好(R2=0.950)。结论 Aβ(1-40)和t-tau在AD组与非痴呆神经疾病组之间差异具有统计学意义,可用于两组间的鉴别诊断。P-tau检测方法稳定,实验室间重复性良好,适于临床开展。更多还原

【Abstract】 Objective To explore the role of biomarkers of Alzheimer’s disease( AD) in differential diagnosis of non-dementia neurological disease. Methods 12 patients with AD and 22 patients with other neurological disorders were tested Aβ(1-40),Aβ(1-42),total tau and phosphor-tau in cerebrospinal fluid specimens by using ELISA method. Results Compared with AD patients,the level of Aβ(1-42) and t-tau in patients with other neurological diseases increased,yet Aβ(1-40) and p-tau decreased. Area under the curve of Aβ(1-40) and t-tau were bigger than other biomarkers with specificity and sensitivity higher than 80%. To get an accurate result,the parallel samples were tested in a German laboratory,then p-tau was confirmed with a consistent result(R2= 0. 950). Conclusion Aβ(1-40) and t-tau in CSF could be used in the differential diagnosis of AD and other neural disorders. The detection method of p-tau is more stable and the results are comparable between different laboratories,so p-tau is more reliable for clinical application.更多还原