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正交实验法优化提取二型鳞毛蕨多糖工艺的研究
Study on the Optimization of Extraction Technology of Polysaccharide from Dryopteris cochleata Using Orthogonal Experiment
【摘要】 以二型鳞毛蕨为试材,通过单因素试验研究了浸提温度、料液比、提取时间和乙醇浓度对多糖提取率的影响,并采用正交实验优化了二型鳞毛蕨多糖提取工艺。结果表明:多糖最佳提取工艺为浸提时间2.5h,料液比1∶40g/mL,乙醇浓度80%,浸提温度80℃,在此条件下,多糖的提取率为2.60%;经该试验优化后的工艺合理,多糖提取率高,品质较好。
【Abstract】 Taking Dryopteris cochleata as material,the effect of extraction temperature,solid-liquid ratio,extraction time and the ethanol concentration on polysaccharide yield were studied by single factor test,and the extraction technology was optimized by orthogonal experiment.The results showed that the best extraction technology of polysaccharide from Dryopteris cochleata was the extraction time 2.5h,the solid-liquidratio 1∶40g/mL,the ethanol concentration 80%and extraction temperature 80℃.Under these conditions,the extraction rate of polysaccharide was 2.60%.The optimized extraction process was reliable,and the extraction efficiency of polysaccharide was higher than normal with good quality.
【Key words】 Dryopteris cochleata; polysaccharide; extraction; orthogonal test;
- 【文献出处】 北方园艺 ,Northern Horticulture , 编辑部邮箱 ,2013年22期
- 【分类号】TQ461
- 【下载频次】70