【摘要】 目的探讨缺氧诱导因子-1α(HIF-1α)在妊娠期糖尿病(GDM)胎盘组织中的表达及其与GDM发病的关系。方法选取妊娠36～41周行择期剖宫产的GDM孕妇44例(GDM组)和同期分娩的正常晚期妊娠孕妇37例(对照组)。采用免疫组化PV-6003两步法和逆转录-聚合酶链反应技术分别检测胎盘组织中HIF-1α蛋白和mRNA的表达。结果两组胎盘组织中均有HIF-1α蛋白的表达,GDM组HIF-1α蛋白的表达明显增强。GDM组胎盘组织HIF-1α蛋白的阳性表达率为86.4%,显著高于对照组的32.4%(χ2=22.51,P<0.05)。GDM组胎盘组织HIF-1αmRNA表达水平为0.88±0.12,显著高于对照组的0.47±0.11(t=15.91,P<0.05)。结论 GDM胎盘组织中HIF-1α蛋白和mRNA高表达可能导致胎盘组织病理形态学的异常,从而参与了GDM的发病过程。更多还原

【Abstract】 Objective To investigate the expression of hypoxia-inducible factor-1α(HIF-1α) in placenta of gestational diabetes mellitus(GDM) and its correlation with morbility of the disease. Methods This study consisted of 44 women of 36—41 weeks of pregnancy with GDM scheduled for caesarean section(GDM group),and 37 healthy pregnant women of normal delivery(control group) of the corresponding time period.The expressions of HIF-1α protein and HIF-1α mRNA in placenta were detected by immunohistochemistry PV-6003 two-step method and RT-PCR,respectively. Results HIF-1α was expressed in the placenta of both groups,but that in the GDM group was much stronger.In GDM group,the expression rate of HIF-1α protein was 86.4%,much higher than 32.4% of that in the control(χ2=22.51,P<0.05).The expression of HIF-1α mRNA in the GDM group was 0.88±0.12 versus 0.47±0.11 of that in the control(t=15.91,P<0.05). Conclusion Overexpression of HIF-1α protein and mRNA in GDM placenta may lead to abnormal pathomorphology of placenta and involve in the pathogenesis of GDM.更多还原