【摘要】 目的:探讨自制简易新装置对小鼠断尾取血的采血效果,建立简易、安全、方便、省时的小鼠采血方法。方法:用废弃的50 ml离心管、纸盒、纱布等制作2个采血装置,并列固定于同一6孔板上,对50只6～8周龄的昆明系小鼠进行重复4次断尾采血。结果:自制的固定装置价格低廉、制作简单、操作灵活,并可同时为2只小鼠采血,采血量可达0.25～0.50 ml,实验中小鼠无伤残、无死亡。结论:自制的小鼠固定装置采血量高、安全性好,是理想的小鼠断尾采血装置。更多还原

【Abstract】 Objective: To investigate the effects of a new simple device for blood collection by tail snipping in mice for providing a safey,convenient and economical method.Methods: The device was made by some hard paper,gauze,and two centrifuge tubes(50 ml),and which were fixed on a plastic plate.The blood of 50 Kunming mice aged 6 to 8 weeks were collected by tail snipping for repeat 4 times.Results: The device was cheap,and easy to be made and handled,which could collect blood for two mice at the same time.No mouse was disabled or dead during the experimental course.Conclusions: The device is a safe and ideal equipment for blood collection by tail snipping in mice.更多还原