【摘要】 目的探讨退变性腰椎滑脱症患者椎间盘组织中TGF-β1和Smad3基因的表达及其临床相关影响因素。方法应用免疫组化染色法及JEDA801D形态学图像分析系统分别对42例退变性腰椎滑脱椎间盘髓核组织及12例正常对照组椎间盘组织中TGF-β1和Smad3表达水平进行测定,正常对照组均系腰椎爆裂性骨折患者椎间盘髓核组织。结果实验组TGF-β1和Smad3累计光密度值分别为(62.79±18.53)、(32.72±13.41),显著高于对照组(39.07±19.76)、(20.95±6.28),两者比较差异有统计学意义(P<0.01);Pearson相关性分析显示实验组TGF-β1和Smad3表达水平呈正相关(r=0.709,P<0.05);多元线性回归分析显示年龄、病程和滑脱分度对两种蛋白表达有重要影响。结论 TGF-β/Smad信号通路参与退变性腰椎滑脱的发生发展过程,调节该信号通路的表达可能为退变性腰椎滑脱症的治疗提供新的途径。更多还原

【Abstract】 Objective To investigate the expression and clinical related factors of TGF-β1 and Smad3 in the intervertebral discs of patient suffering from lumbar degenerative spondylolisthesis.Methods Immunohistochemical staining and morphological image analytical system were used to assay the expression of TGF-β1 and Smad3 in the intervertebral discs of 42 patients suffering from lumbar degenerative spondylolisthesis.12 intervertebral discs harvested from patients with trauma of vertebra were recruited as the control group and were assayed with the same method.Results The levels of TGF-β1 and Smad3 were both significantly higher in the study group(the mean grey numbers of TGF-β1 and Smad3 were 62.79±18.53 and 32.72±13.41,respectively)than in the control group(the mean grey numbers were 39.07±19.76 and 20.95±6.28,respectively,both P<0.01).Pearson correlation analysis showed a positive correlation between expressions in the study group(r=0.709,P<0.05).Multiple linear regression revealed that grade classification of spondylolisthesis,course of disease and age were key factors for TGF-β1 and Smad3 expression.Conclusion The TGF-β/Smad signaling path participates in the occurrence and development process of degenerative spondylolisthesis.Modulating the expression of this path maybe a novel approach to treatment of lumbar degenerative spondylolisthesis.更多还原