【摘要】 目的通过GC/MS分析LEO的活性成分。方法将LEO溶于正己烷,配成2%的溶液,每次进样1μL,流经DB-5毛细管柱,采用GC/MS技术并与NIST标准谱库进行比对确定LEO中各化学成分,按峰面积归一化法得出各组分百分含量。结果 GC/MS分析发现LEO的12种化学成分,占总LEO含量的98.85%,其中含量最高的是m-Mentha-6,8-diene,(R)-(+)-达到77.04%,其次为-Terpinen占14.90%。结论该试验建立的方法简便、快捷、准确,可为EO检测的标准操作流程提供参考,并为建立精油指纹图谱库提供可能。更多还原

【Abstract】 Objective Analysis of lemon essential oil activity component by GC/MS.Methods Gas chromatograph fitted with a DB-5 capillary column;volume injected,1 μL of lemon essential oil in n-hexane(2%).The identification of compounds was based on direct comparison of the mass spectral data with those for the standards and by computer matching with the NIST Library.Component percentage obtains by peak area normalization method.Results The GC-MS analysis of the lemon essential oil led to the identification and quantification of approximately 12 main compounds,which accounted for 98.85% of the total components present.M-Mentha-6,8-diene,(R)-(+)-(77.04%) was the principal component followed by -Terpinen(14.90%).Conclusion The method is convenient,fast and accurate which can become essential oils detection standard operating procedure and provide the possibility to establish fingerprint library of essential oils.更多还原