【摘要】 [目的]克隆1个麻疯树AP2/EFR家族的基因,并构建其植物表达载体。[方法]利用麻疯树EST数据库中的AP2/ERF相关信息设计引物,通过RT-PCR克隆麻疯树AP2/EFR家族的基因,用生物信息学方法对其序列进行分析,并构建其植物表达载体。[结果]试验克隆到1个AP2/EFR家族基因JcERF;从cDNA序列、氨基酸序列、保守域序列、功能组成、理化性质、进化树等方面进行分析,结果显示JcERF属于AP2/EFR家族中的ERF亚家族;将JcERF连接至pCAMBIA1301载体上,构建了以35S为启动子的植物表达载体。[结论]该研究为麻疯树中JcERF的功能研究及提高麻疯树的抗逆性奠定了基础。更多还原

【Abstract】 [Objective] The paper was to clone an AP2/EFR family gene from Jatropha curcas L.and to construct its plant expression vector.[Method]The AP2/ERF related information in J.curcas EST database was used to design primers and the AP2/EFR family genes were cloned by RT-PCR.The sequence of gene was analyzed by bioinformatics methods and its plant expression vector was constructed.[Result] An AP2/EFR family gene JcERF was cloned;the cDNA sequence,amino acid sequence,conserved domain sequence,function composition,physicochemical properties,phylogenetic trees were analyzed,and the results showed that the JcERF gene belonged to the ERF subfamily of AP2/EFR family;the JcERF was connected to pCAMBIA1301 vector and a plant expression vector was constructed with a promoter of 35S.[Conclusion]The study laid a foundation for the function research of JcERF in J.curcas and the improvement of its resistance to stress.更多还原