【摘要】 以无血清的RPMI-1640培养液灌洗奶牛腹腔,分离获取奶牛腹腔巨噬细胞,在含10%小牛血清的RPMI-1640培养液中培养。采用倒置显微镜观察细胞形态,并通过瑞氏染色计算其纯度。结果表明,采用无血清的RPMI-1640培养液灌洗奶牛腹腔,能够获得较高纯度的巨噬细胞,能为奶牛胞内寄生菌存活机制的研究提供很好的试验材料。更多还原

【Abstract】 The peritoneal cavity of dairy cow was douched with RPMI-1640 culture medium without fetal calf serum(FCS),and the peritoneal macrophages were separated and cultured in RPMI-1640 medium with 10% FCS.And then the cell morphology was observed under inverted microscopy,and the purity of macrophages was analyzed by Wright’s staining.The results showed that highly purified macrophages were obtained by this method,which could be prepared for researches on the mechanism of intracellular bacterium living in dairy cow.更多还原