【摘要】 观察奶牛在布鲁氏菌S19疫苗免疫状态下血清凝集抗体的动态变化规律和免疫后奶牛对布鲁氏菌水解素的皮肤迟发性变态反应差异,并探索两者的相关性,寻找选择布鲁氏菌病抗性牛的方法。选择布病阴性的性成熟青年黑白花奶牛50头,注射S19疫苗1×109CFU/头,定期采集血液测抗体水平,第60天用布鲁氏菌水解素皮内注射,检测变态反应。结果显示,所有免疫牛均能在15天时检测到凝集抗体,30天达到峰值。迟发性变态反应表明,免疫牛对布鲁氏菌水解素具有良好的敏感性,变态反应强度与抗体峰值水平存在相关性,但差异不显著,相关系数为0.235。更多还原

【Abstract】 The object of this experiment was to observe the agglutinating antibody variation in serum of dairy cow vaccined with brucella S19,and study the correlation between the agglutinating antibody variation and the brucellin skin allergy.Fifty sexual maturity youth cattle without brucellosis were chosen to be vaccined with S19 in dose of 109 CFU.Blood was collected regularly to check the agglutinating antibody level of the serum.To analysis the skin allergy of the dairy cattle,brucellin was injected intradermally to all the vaccined cattle.The results displayed that the agglutinating antibody could be inspected in serum of all the vaccined cattle on the 15th day and moderately breakdown after a peak on the 30th day.The tardive allergy of the cattle on brucellin showed a favourable sensitivity.The correlation between agglutinating antibody variation in serum and the brucellin skin allergy was not notable with a correlation coefficient of 0.235.更多还原