【摘要】 采用氧化还原法制备氧化石墨烯(GO),通过原位插层聚合法制备出GO与导电聚苯胺(PANI)复合层状纳米材料。采用旋涂法将GO–PANI复合材料旋涂在自组装有十八烷三甲氧基硅烷的硅片上,对核糖核酸(DNA)在复合材料表面上的固定进行电化学性能测试。结果表明,DNA能够很好地固定在GO–PANI复合材料表面上,对将来开发出易于制备的电化学性能优良的DNA芯片提供了实验基础。更多还原

【Abstract】 Graphene oxide(GO)was prepared by method of oxidation reduction process,the nanocomposite of graphene oxide-polyaniline(GO–PANI) via the intercalated polymerization method in situ was constructed.In order to characterize properties of the nanocomposite easily,GO–PANI was spin-coated onto the silicon substrate self-assembled by trimethoxyoctadecylsilane.The electrochemical properties of GO–PANI nanocomposite before and after DNA immobilization/hybridization were detected.It suggests that probe DNA could be immobilized onto the surface of GO–PANI nanocomposite due to the electric static interaction between GO–PANI and DNA in the buffer solution.It could supply the facible method to prepare the electrochemical biosensor with good properties.更多还原