【摘要】 目的:探讨环境危险因素、N-乙酰基转移酶-2(N-acetyltransferase-2,NAT2)基因多态性以及二者的交互作用与女性乳腺癌的关系。方法:采用病例对照研究,选择经病理确诊的原发性乳腺癌新发女性患者140例,对照选择与病例同期入院的非肿瘤女性患者140例,进行饮食习惯与生活方式、环境及职业暴露等问卷调查。采用盐析法提取研究对象静脉血DNA,采用聚合酶链反应-限制性片段多态性(PCR-RFLP)技术检测NAT2基因型并分析环境因素、NAT2基因多态性及其基因-环境的交互作用与乳腺癌的关系。结果:女性乳腺癌的环境危险因素为居住地多环芳烃等环境污染、职业接触多环芳烃、职业使用农药≥10年,室内杀虫剂使用≥5年,被动吸烟≥10年,而烹调使用排油设备、豆类摄入多为保护因素。病例组中NAT2基因快、慢乙酰化表型频率为55.0%、45.0%,对照组为77.9%、22.1%,两组间的差异有统计学意义(P<0.05)。被动吸烟≥10年、职业接触多环芳烃与NAT2慢乙酰化表型存在交互作用,交互作用值分别为3.39和1.70(P<0.05)。结论:被动吸烟≥10年、职业接触多环芳烃与携带慢乙酰化表型存在交互作用,增加了易感个体乳腺癌的发生风险。更多还原

【Abstract】 OBJECTIVE: To explore environmental factors and the association of NAT2 genetic polymorphism and female breast cancer,and their interactions. METHODS:A case-control study was adopted to collect information in 140 female primary breast cancer diagnosed by pathology and treated in major hospitals of Tangshan city. The 140 controls were selected from the female patients without tumor from the same hospitals at the same time. Each subject was investigated by an unified questionnaire,which mainly included eating habits and ways of life,environment and occupational exposure,etc. DNA was extracted by salting out method,and NAT2 genotype were detected by polymerase chain reaction-restriction fragment polymorphism (PCR-RFLP). SPSS 13.0 were adopted to statistically analyze the data. RESULTS:Environmental risk factors of female breast cancer were environmental PAHs pollution of residence,occupational exposure to PAHs,occupational use of pesticide exceeding 10 years,indoor insecticide use over 5 years,passive smoking wore than 10 years. While more intake of beans,fumes exhaust equipment used in cooking were all protective factors. The frequencies of rapid and slow speed acetylation phenotypes were 55.0%,45.0% in case group,and 77.9%,22.1% in control group,respectively. The difference was statistically significant (P<0.05). There were interactions between passive smoking exceeding 10 years,as well as occupational PAHs exposure and NAT2 slow speed acetylation phenotype. The interaction value were 3.39 and 1.70,respectively (P<0.05). CONCLUSION: There were interactions between passive smoking or occupational exposure and carrying slow speed acetylation phenotype,which would increase the risk of breast cancer.更多还原