【摘要】 目的探讨超高效液相色谱(UPLC)测定化瘀止痛片中人参皂苷Rg1和三七皂苷R1含量。方法采用EndeavorsilTM C18(50mm×2.1mm,1.8μm)柱,流动相为乙腈-水(24:76),流速为0.4mL.min-1,柱温为室温,检测波长为203nm,进样量2μl。结果化瘀止痛片中人参皂苷Rg1和三七皂苷R1分别在1.646-82.3μg.mL-1,0.43-21.5μg.mL-1范围内与相应峰面积呈良好线性关系,平均加样回收率分别为100.07%、99.96%,RSD值分别为2.02%、2.13%(n=6)。结论与HPLC法相比,UPLC法在不影响分离效果的情况下可大大提高制剂中三七皂苷R1、人参皂苷Rg1的分析速度,改善分析效果;同时可减少溶剂的消耗。UPLC是可作为替代传统HPLC的一种更为方便、快捷、可靠的测定方法。更多还原

【Abstract】 Objective To establish the quantitative method of ginsenoside Rg1 and notoginsenoside R1 in HuaYu ZhiTong Tablets by ultra performance liquid chromatography(UPLC).Methods This assay was performed on EndeavorsilTM C18(50mm×2.1mm,1.8μm),column with acetonitrile-water(24:76) as mobile phase in gradient elution at a flow rate of 0.4mL?min-1.And the detection wavelength was 203 nm.The column temperature was 25℃.The injection volume was 2μl.Results The Ginsenoside Rg1 and Notoginsenoside R1 in HuaYu ZhiTong Tablets had good linearity within the range of 1.646-82.3μg?mL-1,0.43-21.5μg?mL-1 respectively.And their mean recovery rates were 100.07%,99.96%,with RSD of 2.02%,2.13%(n=6).Conclusion This UPLC method may greatly improve the separation efficiency and analysis speed in the case of notoginsenoside R1 and ginsenoside Rg1 in HuaYu ZhiTong Tablets while reducing the solvent consumption.As an alternative of conventional HPLC,UPLC is more convenient,rapid and feasible.更多还原