【摘要】 目的:观察脾虚大鼠胃粘膜EGFR蛋白表达的变化及补中益气汤对其的影响,并探讨相关机制。方法:SD大鼠称重随机分组,分为设空白对照组、脾虚损伤组、补中益气汤组、非脾虚损伤组。脾虚损伤组、补中益气汤组大鼠给予100%大黄水煎剂灌胃,一天2次,连续10天,第11天补中益气汤组给予补中益气汤灌胃治疗7天,第18天,除空白对照组外的另外三组均灌服消炎痛造成胃粘膜损伤。采用免疫组化法检测EGFR蛋白表达的变化。结果:与非脾虚损伤组比较,脾虚损伤组大鼠胃粘膜EGFR蛋白表达明显下调;与脾虚损伤组比较,补中益气汤组大鼠胃粘膜EGFR蛋白表达明显升高。结论:补中益气汤可提高脾虚大鼠消炎痛攻击后EGFR蛋白表达,促进受损胃粘膜的修复,该方对脾虚大鼠胃粘膜易损性复健作用的机制可能与此有关。更多还原

【Abstract】 Purpose:The expression of gastric mucosa EGFR protein in the Spleen Deficient rats and the effect of Buzhongyiqitang were observed,then the related mechanisms were discussed.Methods:The rats were randomly divided into the spleen deficiency with injury group,The modelof spleen deficiency was copied by Dahuang,and Buzhongyiqitang group,the blank control group,and the non-spleen deficiency with injury group according to their weight.The spleen deficiency with injury group and the Buzhongyiqitang group were administrated by 100% DaHuang solution,twice a day,for 10 days.Then the Buzhongyiqitang group was treated by Buzhongyiqitang for 7 days,twice a day.In addition to the control group,the other three groups were fed with indomethacin solution to cause mucosa injruy after treatment.IHC was adopt to detect the protein expression of EGFR.Results:Compared with the non-spleen deficiency with injury group,the expression of gastric mucosa EGFR protein in the spleen deficiency with injury group was decreased significantly(P<0.01);Compared with the spleen deficiency with injury group,the expression of gastric mucosa EGFR protein in the Buzhongyiqitang group was increased significantly(P<0.05).Conclusion:Buzhongyiqitang increased the expression of EGFR protein greatly,and could activate the downstream of proteins siganl pathways,which may be one of gastric mucosa’s protective mechanisms of Buzhongyiqitang.更多还原