【摘要】 目的用实时荧光定量PCR(FQ-PCR)定量检测不孕不育患者解脲支原体(UU)、沙眼衣原体(CT)基因,观察UU、CT感染情况与不孕不育的关系,探讨实时荧光定量PCR在不孕不育患者诊断和治疗中作用。方法采用FQ-PCR技术检测标本402份。结果 UU阳性率是32.8%,其中男性UU阳性率是21.4%,女性UU阳性率是44.3%。CT阳性率是16.2%,其中男性CT率是11.4%,女性CT率是20.9%。UU感染率在男性、女性患者有极显著性差异(P<0.01),而CT感染率在性别之间有显著性差异(P>0.05)。结论解脲支原体(UU)、沙眼衣原体(CT)是引起男女不育的主要原因之一,实时荧光定量PCR检测UU、CT基因具有操作简单、反应时间短、重复性好、结果客观准确、敏感性和特异性好等优点,其结果对临床诊断、治疗有一定指导意义。更多还原

【Abstract】 Objective: To detect quantitatively the gene of Ureaplasm urealyticum(UU) and Chlamydia trachomatis(CT)In fertility by technology of fluorescence quantitative polymerase chain reaction(FQ-PCR),to understand further infections condition of UU and CT,and to provide clinical treatment evidence to the disease.Methods: Detection 402 speciments by technology of FQ-PCR.Results: the positive of UU was 32.8% of all these;male positive was 21.4%,and female was 44.3%.the positive of CT was 16.2% of all these;male posiaive was 11.4%,and female was 20.9%.Infectious rates of UU、 CT had obvious statistics difference between males and females.Conclusion: The fluorescence quantitative PCR assays showed advantages which were mainpulated simply,time—saving,more extra,more sensitive and more spectific in detecting CT and UU.FQ-PCR had directive meaning for dagnosis and treatmeat.更多还原