【摘要】 目的:建立乳香中3种乙酰乳香酸类成分的HPLC含量测定方法,为全面评价乳香药材质量提供依据。方法:采用HPLC方法测定,Diamonsil(钻石)C8色谱柱(250 mm×4.6mm,5μm);乙腈-0.1%磷酸(A:80:20;B:70∶30)为流动相;检测波长210nm,250nm;柱温35℃。结果:α-乙酰乳香酸、β-乙酰乳香酸、11-羰基-β-乙酰乳香酸分别在0.099～9.9μg(0.999 9)、0.097 6～9.76μg(0.999 9)、0.099 7～9.97μg(0.999 9)范围内呈线性,平均回收率分别为98.6%、101.9%、98.25%,RSD分别为1.38%、1.49%、1.86%(n=6);乳香药材供试品中3种乙酰乳香酸的百分含量分别在0.23%～2.41%、0.45%～4.27%、0.35%～5.27%范围。结论:本方法简便,稳定,可用于乙酰乳香酸类成分的定量分析。更多还原

【Abstract】 Objective:In order to provide scientific evidence for overall quality evaluation of Frankincense,a trial to develop HPLC method for quantitative Analysis of three Acetyl Boswellic Acids in Frankincense were carried out.Methods:The HPLC method was performed on Diamonsil C8 column(250mm×4.6mm,5μm),with acetonitrile-0.1% phospholic acid(A:80∶ 20;B:70∶ 30) as the mobile phase;UV detection wavelength 210nm for α and β-acetyl boswellic,250nm for 11-keto-β-acetyl boswellic acid;the column temperature was 35℃.Results:The liner ranges of α-acetyl boswellic acid,β-acetyl boswellic acid and 11-keto-β-acetyl boswellic acid were 0.099~9.9μg(0.9999),0.0976~9.76μg(0.9999) and 0.0997~9.97μg(0.9999);the average recoveries were 98.6%,101.9% and 98.25%,and RSD were 1.38%,1.49% and 1.86%(n=6);the content ranges of three boswellic acids in Boswellia carterii were 0.23%~2.41%,0.45%~4.27% and 0.35%~5.27% respectively.Conclusion:The method is proved to be convenient and reliable,so it can be used for quantitative analysis of acetyl boswelic acids.更多还原