【摘要】 四氧化三铁(Fe3O4)纳米颗粒可通过水热法一步复合在化学还原氧化石墨烯(CRGO)表面,得到的复合物Fe3O4/CRGO用于固载酶。通过扫描电子显微镜(SEM)、X 线衍射仪、红外光谱仪及振动样品磁强计对Fe3O4/CRGO的表观形貌及结构进行测试和表征。结果显示:Fe3O4纳米颗粒随机分散在CRGO表面,Fe3O4 纳米颗粒的平均直径约 260 nm。利用辣根过氧化物酶(HRP)研究Fe3O4/CRGO 复合物的酶固定化性能。HRP 的饱和固载量为23.3mg/g,固定化后的酶在重复利用10次后依然保留原始酶活性的70%。固载后的HRP显示出更宽的pH耐受范围及很好的重复利用性,表示 Fe3O4/CRGO复合物可作为固载酶的良好基底并具有实际应用价值。更多还原

【Abstract】 A one-step hydrothermal procedure to form Fe3O4 nanospheres on chemically reduced graphene oxide (CRGO) surfaces was proposed, and these nanocomposites were used as substrates for enzyme immobilization. The as-prepared Fe3O4/CRGO nanocomposites were characterized using scanning electron microscopy (SEM), X-ray powder diffraction (XRD), FT-IR and vibrating sample magnetometer. Fe3O4 microspheres are randomly distributed on graphene sheets, and the average diameter of Fe3O4 microspheres is about 260 nm. Horseradish peroxidase (HRP) was used as a model enzyme to investigate the immobilization activity. The HRP loading was 23.3 mg/g supports and retained 70% of the first use after ten cycles. The catalyzed capability of immobilized HRP was investigated and the immobilized HRP exhibited broader pH stability and excellent reusability. The results show that the Fe3O4/CRGO nanocomposites are appropriate for the immobilization of enzyme, and could have potential use in practical.更多还原