【摘要】 目的:建立高效液相色谱法同时测定肝复康丸中五味子醇甲、五味子酯甲、五味子甲素和五味子乙素等4种成分的含量测定方法。方法:采用Agilent TC-C₁₈色谱柱（250 mm×4.6 mm,5μm）;乙腈-水为流动相,梯度洗脱,流速0.8 ml·min^-1;检测波长:254 nm。结果:五味子醇甲、五味子酯甲、五味子甲素、五味子乙素与其相邻杂质峰能完全分离,五味子醇甲、五味子酯甲、五味子甲素、五味子乙素的线性范围（相关系数）分别为68.62～686.25μg·ml^-1（r=0.999 9）,15.54～155.40μg·ml^-1（r=0.999 8）,6.45～64.50μg·ml^-1（r=0.999 9）和15.57～155.70μg·ml^-1（r=0.999 9）。平均回收率分别为100.80%,100.46%,101.06%和100.55%,RSD为1.31%,1.72%,2.00%和1.67%。结论:本方法简便、准确、重复性好,能排除其他成分的干扰,可用于该制剂的质量控制的评价。更多还原

【Abstract】 Objective:To establish the method for determination of schisaiidrin,sehisantheriii,deoxysehizandriii and schisaiidrin B in Ganfukang pills by HPLC.Method:An Agilent TC-C₁₈（250 mm×4.6 mm,5μm） column was used with the mobile phase of acetonitrile-warter with gradient elution.The How rate was 0.8 ml ? min^-1 and the detection wavelength was at 254 nm.Result; Schisandrin,sehisantheriii,deoxysehizandrin and schisaiidrin B eould be completely separated from the impurity peaks.The linear range of those four components was 68.62-686.25μg ? ml^-1（r =0.999 9）,15.54-155.40μg ? ml^-1（r =0.999 8）,6.45-64.50μg ? ml^-1（r = 0.9999） and 15.57-155.70μg ? ml^-1（r = 0.999 9 ）.respectively.The average recovery was 100.80%,100.46%, 101.06%and 100.55%with RSD of 1.31%,1.72%.2.00%and 1.67%,respectively.Conclusion:The method is simple and accurate with good reproducibility and can be used in the quality control of Ganfukang pills.更多还原