【摘要】 目的:建立丹红片中丹参素和原儿茶醛的含量测定方法。方法:采用高效液相色谱法,色谱柱为Diamonsil^TMC₁₈（250 mm×4.6 mm,5μm）,以甲醇-0.05%磷酸水溶液（20:80）为流动相,检测波长为280 nm,流速为1.0 ml·min^-1,柱温:30℃。结果:丹参素钠在8.160～97.920 mg·L^-1的浓度范围内与其峰面积呈良好的线性关系（r=0.999 3）,平均回收率为100.06%,RSD=0.46%（n=6）;原儿茶醛在0.292～2.336 mg·L^-1范围内具有良好的线性关系（r=0.999 1）,平均回收率为99.92%,RSD=0.69%（n=6）。结论:该方法简便易行,重复性好,准确度高,为丹红片的质量控制奠定了基础。更多还原

【Abstract】 Objective:To develop an HPLC method for the determination of Danshensu and protocatechuic aldehyde in Danhong tablets.Method:A Diamonsil^TM C₁₈ column（250 mm×4.6 mm,5μm） was used at 30℃,the mobile phase consisted of methanol-0.05% phosphoric acid aqueous solution（20:80）,the flow rate was 1.0 ml·min^-1 and the detection wavelength was 280 nm.Result: The linear ranges were 8.160-97.920 mg·L^-1（r=0.999 3） for sodium Danshensu and 0.292-2.336 mg·L^-1（r =0.999 3 ）for protocatechuic aldehyde.The average recovery of Danshensu and protocatechuic aldehyde was 100.06%（RSD = 0.46%） and 99.92% （RSD =0.69%）,respectively（n =6）.Conclusion:The method is simple,accurate and reliable with good reproducibility.It can be used as a quality control method for Danhong tablets.更多还原