【摘要】 建立了高效液相色谱(HPLC)测定饲料中三聚氰胺含量的方法。样品中三聚氰胺用三氯乙酸溶液提取,提取离心后经混合型阳离子交换固相萃取柱净化,洗脱物吹干后用流动相溶解,用高效液相色谱仪进行测定。使用EliteMSP5 m(ID4.6mm×150mm)色谱柱,三聚氰胺保留时间为14min,方法验证结果表明该方法具有良好的稳定性和重现性,该方法的三聚氰胺方法检出限为0.046mg/kg,方法定量限为0.153mg/kg,远低于农业部标准NY/T1372-2007的检出限2.0mg/kg,为饲料样品的安全性评价提供了稳定、高灵敏分析方法。更多还原

【Abstract】 A high performance liquid chromatographic method was established for the determination of melamine in feed.The samples were extracted with trichloroacetic acid solution.After centrifuged,the supernatants were cleaned by mixed cation exchange SPE column.The eluant were dried,then redissolved by initial mobile phase.Separation was performed by using Elite MSP column(ID4.6mm×150mm,5μm),the retention time of melamine was 14min.The limit of detection(LOD)of the method was 0.046mg/kg and the limit of quantification(LOQ) was 0.153mg/kg.The method was sensitive and reliable and more propitious to be used for the determination of melamine in feed.更多还原