【摘要】 目的探讨高糖膳食对载脂蛋白AI基因(APOA1)启动子区-75 G/A多态性不同基因型健康青年血脂及载脂蛋白比值的影响。方法给予56名平均年龄为(22.89±1.80)岁的健康青年7 d平衡膳食和6 d高糖膳食。平衡膳食的热量组成为15%蛋白质、31%脂肪和54%碳水化合物;高糖膳食的热量组成为15%蛋白质、15%脂肪和70%碳水化合物。于膳食干预的第1天、第8天和第14天清晨抽取12 h空腹静脉血,测定血脂及载脂蛋白浓度,计算甘油三酯(TG)/高密度脂蛋白胆固醇(HDL-C)、总胆固醇(TC)/HDL-C、低密度脂蛋白胆固醇(LDL-C)/HDL-C及载脂蛋白B100(APOB100)/载脂蛋白AI(APOAI)的比值。提取全基因组DNA,聚合酶链式反应-限制性片段长度多态性法分析APOA1-75 G/A多态性。结果基础值时,血脂及载脂蛋白比值在男性基因型之间的差异无统计学意义(P>0.05);在女性中,A等位基因携带者LDL-C/HDL-C显著高于GG基因型受试者(P<0.05)。高糖膳食后,TC/HDL-C在各性别和基因型分组中均显著降低(P<0.01)。LDL-C/HDL-C在男性各基因型中显著降低(P<0.05);在女性中,LDL-C/HDL-C仅在A等位基因携带者中显著降低(P<0.01),在GG基因型受试者中差异无统计学意义(P>0.05)。结论 APOA1-75 G/A多态性A等位基因可能对其女性携带者的LDL-C/HDL-C比值有一定的影响。更多还原

【Abstract】 Objective To investigate the effects of a high-carbohydrate diet on the lipid and apolipoprotein ratios in healthy young adults with different genotypes of the polymorphism at-75 site in the promoter region of the gene of apolipoprotein AI(APOA1).Methods Fifty-six subjects aged(22.89±1.80) years were given a wash-out diet for 7 days,followed by a high-carbohydrate diet for 6 days.The wash-out diet contained 15% protein,31% fat,and 54% carbohydrate.The high-carbohydrate diet contained 15% protein,15% fat,and 70% carbohydrate.Twelve-hour fasting serum lipids and apolipoproteins B100 and AI were measured on the mornings of the 1st,the 8th,and the 14th days from the beginning of the wash-out diet.The ratios of triglyceride(TG)/high-density lipoprotein cholesterol(HDL-C),total cholesterol(TC)/HDL-C,low-density lipoprotein cholesterol(LDL-C)/HDL-C,and apolipoprotein B100(APOB100)/apolipoprotein AI(APOAI) were calculated.The genome DNA was extracted and the polymorphism of APOA1-75 G/A was determined by polymerase chain reaction followed by restriction fragment length polymorphism assay.Results At baseline,the lipid and apolipoprotein ratios showed no significant differences between the GG genotype and the A carriers in males(P>0.05),whereas the female A carriers had a significantly higher ratio of LDL-C/HDL-C compared with the female subjects with the GG genotype(P<0.05).Following the high-carbohydrate diet,significant decreases of TC/HDL-C were found in all the groups,regardless of sex and genotype(P<0.01).LDL-C/HDL-C experienced significant decreases in both the genotypes in males(P<0.05),while in females,significant decrease of LDL-C/HDL-C was only observed in A carriers(P<0.01).Conclusion The A allele of the-75 G/A polymorphism in APOA1 may have specific effects on the LDL-C/HDL-C ratio in females.更多还原