【摘要】 目的观察土贝母皂苷Ⅱ对诱导性肝癌大鼠肝细胞周期的影响。方法以诱导性肝癌大鼠为材料,采用流式细胞仪检测土贝母皂苷Ⅱ对诱导性肝癌大鼠肝细胞DNA含量,并分析细胞周期。结果经土贝母皂苷Ⅱ治疗的动物肝S期细胞较模型组明显减少,而G1/G0期细胞增多,几乎恢复到正常水平。结论土贝母皂苷Ⅱ可以阻止肝细胞从G1期向S期进程,使DNA合成受到抑制和阻断,造成G1期细胞的堆积。更多还原

【Abstract】 Objective: To observe the influence of saponin Ⅱ in rhizoma bolbostemmatis on cell cycle of liver in rats with inducibility carcinoma.Methods:The DNA level of liver in rhizoma bolbostemmatis was detected by the flow cytometry in order to anlysis the cell cycle of it.Results: The concentration of S phase cell was reduced after intervention by saponin Ⅱ in rhizoma bolbostemmatis.Meanwhile,the level of G1/G0 phase cell was increased.Conclusion: After the process of G1 phase to S phase could be blocked by the saponin Ⅱ in rhizoma bolbostemmatis,the DNA synthesis is inhibited and blocked so that the G1 phase cell is accumulated.更多还原